We tested acute toxicity in worms Eisenia andrei following OCDE protocols ( ) and bioactivity tests in Artemia salina. The alkaloids fraction. Bioensayo de toxicidad en artemia salina. Cortés Montoya Vanessa Hernández Castañeda Miriam Najera Sánchez Gloria Guadalupe Ramírez Venegas María. Bioensayo de La Toxicidad Del Nitrato de Potasio en Reproductores de Artemia Salina en Condiciones de Laboratorio – Download as Word Doc .doc), PDF.

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PhD thesis, University of Vigo, pp. However, Romano et al. On the other hand, A.

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Toxin variability in cultured and natural populations of Alexandrium tamarense from southern South America — Evidences of diversity and environmental regulation. For Artemia salina toxicity assay, the five most active were all from the 2nd and 3rd collections. Editorial Universidad del Valle; After that time, all larvae had moulted to instar II-III stages and the hatched nauplii were separated from remaining cysts using a Pasteur pipette.

By the Km value, the physiological concentration of complex carbohydrates in the newly hatched Artemia nauplii was near the value equivalent to 0. Other standardized toxicological assays using more distant taxonomic groups are also available and have been applied extensively Ribo and Kaiser Ribo J. The highest concentration of the dinoflagellate, cell mL —1caused the inhibition of the embryonic development in all of the sea urchin larvae while from the lowest concentration tested cell mL —1to cell mL —1 no significant differences from the control were detected Fig.

Effects of toxic Alexandrium species on the survival and feeding rates of brine shrimp, Artemia salina. After incubation, one replicate per treatment was used to measure oxygen, salinity and temperature in order to ensure normal optimal conditions.


The cytotoxicity effect was tested using brine shrimp. Skip to main content. These investigators observed that Prymensium cells were attached to most exterior surfaces, to fish gills and to feeding and respiratory appendages in zooplankton, which contributed to the movement depletion of individuals.

A set of ecotoxicological tests was carried out to investigate the effects of A. Comparative study of the life cycles of Alexandrium tamatum and Alexandrium minutum Gonyaulacales, Dinophyceae in culture. Toxicity may be variable between strains of potentially toxic species e. The active extracts of D. Our results agree with those of Zhenxing et al. We then made a qualitative chemical identification of hydrolysable tannins and condensed tannins. Toxic blooms cause negative impacts and economic losses in many parts of the world and their consequences and mechanisms depend on the species involved.

Imprenta Nacional de Colombia; The toxicity was investigated using two Gram positive bacteria Bacillus subtilis and Staphylococcus aureus, and two Gram negative bacteria Escherichia coli and Pseudomonas aeruginosa.

Wound-activated chemical defense in unicellular planktonic algae.

Artemia salina Research Papers –

On the other hand, the filtrate of A. The mortality observed in this initial experiment helped us to define a more precise concentration range for a definitive test see Table 1 for concentration ranges. For strain isolation, a single cell was captured with a glass capillary under an inverted microscope Nikon Diaphot TMD. The toxicity was investigated using the two Gram positive bacteria Bacillus subtilis and Staphylococcus aureus, and two Gram negative bacteria Escherichia coli and Pseudomonas aeruginosa.

Therefore, the minimum toxic concentration values established in the present study for the model organisms cannot be considered as a real toxic value. Because of these deleterious effects, several methods for determining the toxicity of toxxicidad microalgae have been used to date.


Due to the high toxicity reported for A. In the present study, adult amphipods were not sensitive to the toxicity of A. Topics Discussed in This Paper. The result of the filtrate reported in the present study should therefore be considered carefully. Related to the filtrate of A.

Artemia salina

Its life cycle begins with the hatching of dormant cysts that, once rehydrated in salt water, start the development as nauplii larvae, which are the material for the bioassays. The procedure followed in this study to carry out the Paracentrotus lividus embryo-larval bioassay, was taken from the protocol of Garmendia et al. In vivo drug discovery in the zebrafish.

Among the genera responsible for harmful algal blooms HABsthe genus Alexandrium is one of the most important, in terms of severity, diversity and distribution of bloom impacts Anderson et al. Differences were considered as significant when p was lower than 0. Retos ante el tratado de libre comercio.

Menchaca Menchaca I. Antioxidant evaluations showed three of twelve were active and two were considered moderately active, with a relationship dependently of concentration. The Michaelis-Menten constant Km for starch of the Artemia nauplii amylase was estimated using Lineweaver-Burk plot to be 0. The effect of exposure time can be observed with LC 50 values at 24 and 48 h: Bioactive furanonaphthoquinones from Crescentia cujete.